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 Softmax Pro GxP Software 6molecular devices spectramax m5 Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory

The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Compatible with SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3 readers 0200-6191: Gray replacement validation plate case Compatible with. We also offer depot Repair Service from our base of operations in Southern California (San Diego). 312 gain calibration failed. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 72 MB. It has a cuvette port, spectral scanning, and up to six wavelengths per read. Wavelength precision. es •. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. All other trademarks or registered trademarks are the property of their respective owners. Using a cell-based standard curve and a five-minute incubation, we observed a limit of. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Molecular Devices. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. SpectraMax® ABS. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Representative data from three different SpectraMax Quant dsDNA Assay Kits. Supplier: Molecular Devices. 2018. #R7159) APPLICATION NOTE 如何使用SpectraMax M5 多功能微孔. MOLECULAR DEVICES SpectraMax M5 is an advanced multi-mode microplate reader which combines fluorescence intensity, fluorescence polarization, luminescence, absorbance and time-resolved fluorescence measurements into one integrated system. and California) iv SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual. StakMax Microplate Handling System. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. It has a cuvette port, spectral scanning, and up to six wavelengths per read. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 1. Easy workflow The SpectraMax Quant dsDNA Assay Kits are specifically optimized for use on a microplate reader with preconfigured protocols providedthese roadblocks, Molecular Devices has developed the StakMax® Microplate Stacker. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. 6. 2. Save the results of the Protocol in a data (. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Plate stacker and robot integration. SpectraMax M5, Analyst HT or Analyst GT. It has. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex. i3, M3, M4, M5, M5e, SpectraMax L, FlexStation 3 0200-6186: Cuvette Absorbance Validation Set SpectraMax. 01) + 0. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Molecular Devices Spectramax M5 Microplate Reader; Related products. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. These robust readers have been placed in labs from. 2. 2. ©2013 Molecular Devices, LLC • 8/13 • Printed in USA • PN: 0120-1521. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and well-area scanning. Ferguson, Ph. IMAP technology is based on. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). ® reader to the computer. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Recertification is recommended every two years. Pleasanton, CA, USA. D 1. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Products : SpectraMax M5/M5e reader Fluorescence-based mitochondrial function analysis (PDF) Summary: Mitochondrial dysfunction is implicated in numerous disease states and is also a major mechanism of drug-induced toxicity. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)SpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices) Methods Cell preparation and analysis. FlexStation® 3 Multi-Mode Microplate Reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 7°C and the bottom to 36. View the Molecular Devices websiteMolecular Devices’ SpectraMax® L Luminescence Microplate Reader is a dedi-cated reader with an extended dynamic range of more than 8 orders of magnitude. Lift up the lid over the chamber to insert or remove a cuvette. Turn on Plate Reader and wait. Description 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. minutes and begin reading microplates with seven mouse clicks. Download PDF. These robust readers have been placed in labs from Antarctica to. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 3. Figure 2. SpectraMax M5 reader SpectraMax M5eThe integration time suggested is 5-10 sec. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Molecular Devices. Fortunately, Molecular Devices offers a system with variable bandwidth that can be specific and sensitive at the same time and is suitable for assay optimization, so you do not have to choose. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. SpectraMax M Series (Molecular Devices) FlexStation 3 (Molecular Devices) Fluorescence Microplate Readers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Softmax Pro GxP Software 6. It does not work as expected. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. We therefore gathered salient. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. The ratioof the two fluorescence intensities 665/620 (acceptor/donor) enables the calculation of Delta F (%)Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. 5577. We would like to show you a description here but the site won’t allow us. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. 4. 1. 6% for the on-board optics (monochromators). Note: In this user guide, all references to SpectraMax Multi-Mode. industry-leading data acquisition and analysis tool SoftMax Pro, the SpectraMax i3x Detection Platform is the total solution for all your research needs. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : Multimode Analysis. Select the Driver tab. The single mode Luminescence Readers (LMax II, SpectraMax L), with a Lower Limits of Detection (LLD) use six different light intensities. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. More Product Information. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre-configured protocol provided for customers using Molecular Devices’ SoftMax. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 1. Multimode Readers Comparison Chart. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Cells were treated with increasing concentrations of the antagonist atropine and assayed for response to addition of an EC80 concentration of the agonist carbachol. 635. S. #6590-0128) • Set of 2 Fluorescence Polarization Filters 535nm BW 25nm Polarized Vertical & Horizontal (Molecular Devices cat. This document is provided to customers who have purchased Molecular Devices, Inc. com A. To update the driver. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Description: Molecular Devices ’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. The M5 has two PMTs. D 1 1. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 2. moleculardevicescom Molecular Devices C All rigts reserved SpectraMax Quant dsDNA Assay Kits Optimized DNA measurement for your microplate reader assay DATA SHEET Quantitation of DNA is a critical step in molecular biology requiring accuracy, reliability, and the use of increasingly smaller sample volumes for applications such as next- BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. $2,264. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. Description 1. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 00 USD. Comparing FLIPR Calcium 6 and 6-QF to other dyes shows that both had the largest signal window. The system. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. SpectraMax Injector Cartridge with SmartInject™ Technology. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator 302 low ref light. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Spectral Range. SpectraMax ® M2 Multi-Mode Microplate Reader. Also refer to this Multimode Reader Comparison Chart. FLIPR Calcium 6 and 6-QF Assay Kits provide the largest signal window. Table of Contents. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Molecular Devices SpectraMax M5 Plate Reader Repair. 1 ng/mL to 50 pg/mL (r2 = 1. View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seWe would like to show you a description here but the site won’t allow us. The SpectraMax M5 Multi-Mode Microplate Reader measures UV and visible absorbance, fluorescence, luminescence, TRF and HTRF. In most cases we will be able to perform the repair as listed, however in rare instances, you may be required to ship the instrument into our Service Depot Facility. Prepare your iD series instrument for shipment. in Molecular Devices microplate luminometers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Fully optimized assays for SpectraMax iD3 reader save time on assay development. D. 4. See SpectraMax® Microplate Reader User Manual Download Page. #0200-7011) Instrument setupSoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 7°C (gradient heating design). 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. To evaluate the amount of miR-148b mimic released by the retro Diels-Alder reaction (Fig. This manual is subject to change without notice. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The system provides automation for up to 50 The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. system setup and calibration are controlled from within softmax ro psoftware. -39. 000). AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. HEALTH AND SAFETY CONSIDERAIONS The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175) Sunnyvale, CA - May 17, 2011 - Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the M5e Reader will be the first microplate reader to be used in space. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 3. Home; Knowledge BaseMolecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. cuvette port for assay development • Dual monochromator tunability • Automated absorbance pathlength correction The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dualmonochromator microplate reader. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-SpectraMax® Microplate Reader Suite of Products Knowledge Base. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Photometric accuracy. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. FlexStation™ II. -54. Microplate Spectrophotometers. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. If values are present, then. Molecular Devices. 1) SpectraMax Glo Steady Luc Reporter Assay (6. SoftMax Pro Software can collect data from one or more microplates orSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. measure the depth (optical pathlength) of samples with no temperature. HTRF-compatible SpectraMax Multi-Mode Readers. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. AT&T Manuals. Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. MaterialsThe calculated LOQ values were 0. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. 1 or greater versions. Molecular Devices’SpectraMax ® Microplate Readers have been validated for Transcreener ADP. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). begin reading microplates with seven mouse clicks. #6590-0137) • SpectraMax i3x Multi-Mode Microplate Reader • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 6. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 1 0. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 304 signal level saturation. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi-ModeMicroplateReader SpectraMax® SpectraMaxLMicroplateReader For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way? SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. i3x. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Lck kinase assay with TAMRA-labeled peptide on. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. peptide and phosphopeptide, read on SpectraMax M5 (quadratic curve fit). DRAWER button Opens and closes the microplate drawer when the instrument is in idle state. Wide range of applications; Five preferred detection modes;The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Future ready The SpectraMax i3 x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. View the Molecular Devices websiteMolecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. M2. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. . Table1-1 SpectraMax ® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 7. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. – ®SpectraMax M4 and M5 Multi-Mode Microplate Readers (cat. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. SpectraMax M-series Microplate readers. 9000-0756. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This problem has been remedied by the introduction of Molecular Devices PathCheck Technology, which enables determination of the pathlength in each well of a microplate and automatically normalizes the absorbance value to a 1-cm pathlength. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Results Luciferase emission spectrum. View the Molecular Devices website. Feb 22, 2023Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 3. Unit successfully connected to software, and passes self tests. #R7309) 。标记FAM-p34cdc2来源的磷酸化多肽 校正试剂(Molecular Devices Cat. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Assay Performance with Various Instrument. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. 2. Description 1. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. We would like to show you a description here but the site won’t allow us. 3: The cuvette chamber. 5. Sunnyvale, California -- October 5, 2017. 4. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Additional information is also available in the User Manuals. The. View the Molecular Devices website. View the Molecular Devices websiteThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Table of Contents. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Detection modalities include absorbance (UV-Vis Abs)The chart below provides general information about the availability of on-site and depot repair options. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance X X X X For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Cavicide (Warehouse, Cat # 79300360) 6. 305 reference level saturation. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Description 1. The opaque walls prevent stray light from being detected from neighbouring. Molecular Devices Spectramax M 5 Manual -. Fluorescence Microplate Reader Comparison Chart. Configurable readers with triple-mode cuvette ports, validation tools, and compliance softwareProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e reader. Restart the system. ECvalues were within one-half log and Z factors at EC. Lower limits of detection for ATP determined using the ATPliteThe Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 3 or greater. It has a dual monochromator optics, a compact design, and a touchscreen interface for easy data acquisition and analysis. Published Dec 16, 2020 | Updated Dec 16, 2020. We would like to show you a description here but the site won’t allow us. • Tel. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. substrates using the SpectraMax M5 microplate reader and SoftMax® Pro Software. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. STAKMAX. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. SpectraMax M5. Non-transfected HEK cells were included as controls. The guide also covers the software features, the specifications, and the accessories of the systems. , contact Molecular. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. StakMax Microplate Handling System. Note: In this user guide, SpectraMax ® refers to several SpectraMax . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orMolecular Devices. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Features. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. ; SoftMax® Pro 7. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The dual monochromator optics allow. By utilizing the optimized instrument settings suggested within this Application Note, Z' values > 0. Digitizer Systems. The SpectraMax® M5/M5e Multimode Plate Reader is a high-performance microplate reader that can read volumes as low as 2uL and perform various detection modes for bioresearch and drug discovery applications. 2. F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. 100 readings per well gives optimal Z’ values for this assay. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. This integrated system provides users with a multi. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Qualify the luminescence performance of the SpectraMax . Instrument. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. With optimized reagents, option to operate the. D 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The Multi-mode readers use four different light intensities for Detection Estimates, Magnitude 3 through 6. Sensitive fluorescent quantitation of DNA with the Quant-iT. LoadingIMAP® Technology from Molecular Devices enables rapid, non-radioactive assay of a wide array of kinases and is suited to both assay development and high-throughput. for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. The built-in micro-volume sample port allows for working with sample volumes as small as 0. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software. To support these assays, we provide application notes as well as. SpectraMax M 系列多功能微孔板读板机(酶标仪)可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. The drawing shows a standard microplate of 15 mm height covered with a lid. view more . Published Dec 16, 2020 | Updated Dec 06, 2021. spr SpectraMax Plus 384. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 72 MB. -43. After running the LM1 Plate protocol, not all values in the values in Results sections are displayed; what should I do? This is a SoftMax Pro software problem rather than anything specific to the LM1 plate, protocol or reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax Plus 384 measuring instruments pdf manual download. Molecular Devices. Table of Contents. 2. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Table 1. Anne T. The system provides automation for up to 50The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Choose cables that meet the requirement described in this appendix. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Step 4: Incubate at room temperature for 1 hour, protected from light. 2. In the M2, M5 and Plus. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. View the Molecular Devices websiteLearn how to compare dose-response curves where there is no direct measurement of a product using Parallel line analysis - a new feature from SoftMax Pro 7 software. Both microplate and cuvette ports can be qualified. We would like to show you a description here but the site won’t allow us. 2. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. 5. EUROPE. NORTH AMERICA. 19.